PGGT、PGGT单克隆抗体和GGT mRNA表达在胰头癌诊断中的价值

The diagnostic value of the expression of PGGT,PGGT Mab,GGT mRNA in pancreatic head cancer

目的 :探讨PGGT、PGGT单克隆抗体和GGTmRNA表达在胰头癌诊断中的价值。方法 :采用改良DEAESephadexA50 离子交换柱层析法、PGGT单抗Elisa间接混合夹心法、RT PCR等方法对 14 2例黄疸患者血清PGGT、TGGT及组织中GGTmRNA的表达进行研究。结果 :以血清PGGT >4U/L、PGGT/TGGT >0 .1作为诊断胰头癌的指标 ,其阳性率为 6 2 .5 %。PGGT单克隆抗体检测血清中PGGT恶性阻塞性黄疸及胰头癌阳性率分别为 5 9.0 %及 10 0 %。通过RT PCR法检测GGTmRNA的相对表达量 ,胰头癌组 (占 6 2 .5 % ) >胆管癌组(占 35 .8% ) >正常组 (占 2 9.4 % )。结论 :PGGT、PGGT/TGGT、PGGT单克隆抗体及GGTmRNA表达在梗阻性黄疸鉴别诊断中有其重要意义 ,有望成为诊断胰头癌的方法。

Objective:To explore the diagnostic value of the expression of PGGT,PGGT monoclonal antibody(Mab) and GGT mRNA in pancreatic head cancer.Methods:The expressions of PGGT and TGGT in serum and GGT mRNA in tissues from 142 jaundice patients were measured by modified DEAE Sephadex A 50 ion exchange chromatography(IEC),PGGT Mab ELISA indirect mixed sandwich technique and RT PCR,respectively.Results:The positive rate of pancreatic head cancer was 62.5% by detecting PGGT,TGGT in serum,the cut off value being PGGT>4 U/L and PGGT/TGGT>0.1. When PGGT Mab was used to detect PGGT in patients' serum,the positive rate of malignant obstructive jaundice and pancreatic head cancer went to 59.0% and 100%,respectively. The relative expression of GGT mRNA by RT PCR were respectively 62.5% in pancreatic head cancer,35.8% in cholangiocarcinoma and 29.4% in normal tissue.Conclusion:The significant value of the expression of PGGT,PGGT/TGGT,PGGT Mab and GGT mRNA in the differential diagnosis of obstructive jaundice indicates that they could be a potential method for the diagnosis of pancreatic head cancer.