胃癌P-耐药糖蛋白和P_(53)、mdm2蛋白表达关系的研究

Evaluation of correlation between mdm2 protein,P_(53) protein,P-glycoprotein expression in gastric carcinoma

目的 :探讨P -耐药糖蛋白 (P -gp)和P53 、mdm2蛋白在胃癌组织中的表达关系。方法 :应用免疫组化SABC法对 79例胃癌进行P -gp、P53 及mdm2蛋白表达的检测。结果 :P -gp、P53 及mdm2蛋白在胃癌组织中的阳性率分别为 4 0 5 % (32 / 79)、5 8 2 % (46 / 79)、15 2 % (12 / 79) ;P -gp表达与淋巴结转移有关 (P <0 0 1) ,与胃癌的组织学类型和浸润深度无关 (P >0 0 5 ) ;P53表达与胃癌的浸润深度和淋巴结转移有关 (P <0 0 5 ) ;mdm2与胃癌的组织学类型、浸润深度及淋巴结转移无关 (P >0 0 5 )。P-gp过表达与P53 过表达呈正相关 (P <0 0 1) ,与mdm2过表达呈正相关 (P <0 0 5 )。结论 :P -gp、P53 及mdm2蛋白多并存于胃癌组织中 ,可为临床判断病人耐药程度。

Objective:To study the clinical significance of mdm2,P 53 protein and P-glycoprotein(P-gp) expression in gastric carcinoma tissues.Methods:79 cases of gastric carcinoma was analyzed by SABC immunohistochemical staining.Result:mdm2,P 53 protein and P-gp were detected in 15 2%,58 2% and 40 5%,respectively.P 53 was found correlating with invasive depth of lymph node metastases(P<0 05).There was significant association between P 53 and P-gp positive expression(P<0 05).P-gp expression was significant higher in mdm2,P 53 positive than in mdm2,P 53 negative tumors(P<0 05).Conclusion:Co-expression of mdm2,P 53 protein and P-gp is common,which may offer objective gist for clinical chemoresistance and for prognosis in gastric carcinoma.