薄层扫描法测定牡蛎软体中牛磺酸的含量

Determination Contents of Taurine in Oyster Soft Tissue by TLCS

目的 建立牡蛎软体中牛磺酸的含量测定方法。方法 采用薄层扫描法 ,以乙醇 -水 (7∶ 3)为展开剂 ,展距 8cm;以 0 .2 %茚三酮乙醇液为显色剂 ,10 5℃加热至斑点清晰 ;反射法线性扫描 :λs=5 19nm,λR=6 18nm ,狭缝 :10 .0× 0 .4 m m ,Sx=3。结果 牛磺酸能与其他氨基酸相分离。结论 方法简便、准确、快速。

Objective To establish the method for determination the content of taurine in oyster soft tissue.Methods TLCS is used.Results Taurine can be separated from the other amino acid.Conclusion This method is simple, rapid, sensitive and reproducible.