两种不同凝胶柱测定壳聚糖分子量结果的比较

Comparison of Two Different Gel Columns in Determining Molecular Weight of Chitosan

凝胶渗透色谱法测定壳聚糖分子量 ,以葡聚糖为标准品 ,用 2种不同的凝胶柱 (PLaquagel- OH柱和 TSK G4 0 0 0 PWXL柱 )测定 4种样品的分子量及其分布 ,并经过普适标定。比较结果发现 :PL aquagel- OH柱效果稍好于 TSK G4 0 0 0 PWXL柱。两柱所得重均分子量平均偏差为 0 .17%～ 3.38% ,数均分子量的平均偏差为 0 .90 %～ 3.5 7% ,分子量分布宽度指数 1.34～ 1.5 4。

The molecular weight of chitosan and its distribution were determined by GPC with two different gel columns(TSK column and PL aquagel OH column). T series dextran was used as standard samples and universal calibration was conducted. The comparison shows that the PL aquagel OH column is better than the TSK column,the average deviations of average M W′ are within 0.17%～3.38%, the average deviations of average M n are within 0.90%～3.57%, and the distribution coefficient is within 1.34～1.54.