原发性高血压合并脑梗塞患者血管紧张素Ⅱ-1型受体基因多态性的研究

Influence of angiotensin Ⅱ type 1 receptor gene polymorphism on patients with essential hypertension complicated by brain infarction

目的 探讨血管紧张素 - 1型受体 (angiotensin type1receptor,AT1R)基因 A116 6 /C多态性与原发性高血压及合并脑梗塞的关系。方法 应用聚合酶链反应 -限制性片段长度多态性方法检测70名健康人、72例原发性高血压无合并症患者及 70例原发性高血压合并脑梗塞患者的 AT1R基因型 ;生化技术测定血脂水平。结果 原发性高血压无合并症组及合并脑梗塞组的 C等位基因频率分别为 11.1%和 10 .7% ,均显著高于正常对照组的 3.6 % (P<0 .0 5 ) ;而两者之间的 C等位基因频率差异无显著性 (P>0 .0 5 ) ;原发性高血压患者血浆脂蛋白 a水平与 AT1R基因正相关。结论 提示 AT1R基因可能是原发性高血压的重要遗传因素 ,但与高血压病患者是否易患脑梗塞无关。

Objective To identify the A1166/C polymorphism of angiotensin Ⅱ type 1 receptor (AT1R)gene in patients with essential hypertension complicated with brain infarction (BI). Methods AT1R genotyping with polymerase chain reaction-restrictive fragment length polymorphism was performed in 70 normotensive subjects,72 hypertensive patients without cardio-cerebrovascular diseases(EH-NCCVD) and 70 hypertensive patients with BI. The relationship between the polymorphism of AT1R gene and plasma lipid levels was also studied. Results The frequencies of C allele in the two groups of hypertension were higher than that in the health controls, respectively ( P <0.05). But there was no significant difference in the frequency of C allele between the two groups of hypertension. A positive correlation was observed between the lipoprotein(a) and AT1R gene A1166/C polymorphism in hypertensive patients. Conclusion AT1R gene contributes to the development of essential hypertensive, but not to the incidence of BI in the hypertensive.