野生型p53基因转染卵巢癌SKOV-3细胞对化疗药物顺铂敏感性的影响

Effects of wild-type p53 gene on the chemotherapy sensitivity of ovarian cancer SKOV-3 cells to cisplatin

目的 研究野生型 p5 3基因转染卵巢癌 SKOV- 3细胞对化疗药物顺铂敏感性的影响。方法用脂质体介导的转染技术 ,将含有全长人野生型 p5 3c DNA的真核表达重组质粒分别导入受不同浓度顺铂作用的 SKOV- 3培养细胞中 ,观察 p5 3不同状态下对肿瘤细胞化疗敏感性的差异。结果 转染 p5 3基因后的 SKOV- 3细胞集落形成率与未转染的 SKOV- 3细胞相比 ,抑制率为 5 6 .4%。用 0 .5 μg/ml顺铂分别作用 2 4h、48h后 ,集落形成数分别下降了 76 .2 %、84.1% ;用 1μg/ml顺铂分别作用 2 4h、48h后 ,集落形成数分别下降了 89.5 %、93.7%。转染 p5 3基因后 ,肿瘤细胞 S期和 G2 /M期的比例下降 ,G1 /G0 期的比例增加 ,p5 3基因的转染使卵巢癌细胞发生 G1 期阻滞。结论 外源性野生型 p5 3基因的转染增加了卵巢癌SKOV- 3细胞对化疗药顺铂的敏感性。

Objective: To assess the effect of wild-type p53 gene on the chemotherapy sensitivity of ovarian cancer SKOV-3 cells to cisplatin. Methods: Recombinant eukaryotic expression vector pcDNA3 containing full-length human wild-type p53 cDNA was introduced by lipofectamine-mediated gene transfection into SKOV-3 cultured cells which were acted on by cisplatin of different concentrations. The chemotherapy sensitivity of tumor cells with different-status p53 was observed. Results: The inhibitive rate of formation of clones after p53 cDNA transfection was 56.4% compared with the untransfected one. The formation of clones decreased by 76.2% and 84.1% respectively after being acted on by 0.5μg/ml cisplatin for 24 hours and 48 hours respectively. The formation of clones decreased by 89.5% and 93.7% respectively after being acted on by 1 μ/ml cisplatin for 24 hours and 48 hours respectively. After the introduction of p53 cDNA, the S phase and the ratio of G2/M phase of tumor cells decreased, and the ratio of G1/G0 phase increased. The introduction of p53 gene into cells led to cell cycle arrest in G1 phase. Conclusion: The exogenous introduction of wild-type p53 cDNA into ovarian cancer SKOV-3 cells increased the chemotherapy sensitivity to cisplatin.