瘦素对自发性高血压大鼠血管平滑肌细胞增殖的影响

Effect of leptin on the proliferation of vascular smooth muscle cells derived from spontaneously hypertensive rat

目的 探讨leptin对自发性高血压大鼠血管平滑肌细胞的增殖影响 ,并研究leptinb型受体在VSMC中的表达。方法 本文主要采用培养的自发性高血压大鼠 (SHR)血管平滑肌细胞 (VSMC) ,研究Leptin受体b(OB Rb)的mRNA的表达 ,以及对平滑肌细胞增殖的影响。结果 OB Rb的mRNA仅在SHR大鼠的平滑肌细胞表达 ,而没有在正常对照大鼠 (WKY)的平滑肌细胞中表达。Leptin仅仅可以刺激SHR大鼠VSMC的DNA合成率的增加 ,而对WKY大鼠的VSMC则无作用。同样 ,Leptin对VSMC细胞增殖的作用 ,也仅仅在SHR大鼠可以发现 ,而WKY大鼠则没有。结论 上述研究的结果显示 ;高血压时 ,Leptin是一种对血管平滑肌细胞的增殖因子 ,也许在高血压的发生、发展过程中起重要的作用。

Objective This study was designed to characterize the long form of of leptin (OB Rb) receptor mRNA expression and the effect of leptin on the proliferation of vascular smooth muscle cells (VSMC) derived from SHR and WKY rats.Methods The expression of OB Rb mRNA was measured in VSMC from SHR and WKY using RT PCR. The proliferative effect of leptin on VSMC was investigated by thymidine incorporation and the growth of VSMC. Results OB Rb receptor mRNA was found only in VSMC from SHR, but not in that from WKY. Leptin stimulated the proliferation of VSMC only in SHR, but not in WKY. Conclusion These results demonstrated that leptin is a proliferative factor for VSMC in SHR, implying that leptin may play a promoting role in the development of hypertension.