不同强度恒磁场对人脐动脉平滑肌细胞增殖的影响

Effects of constant magnetic fields of different intensities on the proliferation of human umbilical arterial vascular smooth muscle cells

目的 :探讨不同强度恒磁场对离体人脐动脉平滑肌细胞 (VSMC)增殖的影响。方法 :用含 10 0 ml· L- 1 新生牛血清及成纤维生长因子 (5μg· L- 1 )和表皮生长因子 (10μg· L- 1 )的 DMEM的培养液 ,培养人 VSMC至 3～ 10代 ,将培养液与 VSMC的混悬液 (细胞浓度为 1× 10 5· m L- 1 ) 10 0μL加入 96孔培养板中培养 ,对照组持续培养 72h,实验组 2 4h后将培养板置于 0 .5～ 6 0 m T的恒磁场中继续培养 48h,用 MTT法和 3 H- Td R掺入法检测两组VSMC的增殖数量 (A值和核素闪烁计数值 )。结果 :在恒磁场的作用下 ,0 .5 ,1,2 ,5 m T组人脐 VSMC的增殖数量显著低于对照组 (P<0 .0 5 )。 10 ,30 ,6 0 m T组人脐 VSMC的增殖数量显著高于对照组 (P<0 .0 1)。结论 :恒磁场强度为 0 .5 ,1,2 ,5 m T时抑制 VSMC增殖 ,10 ,30 ,6 0 m T时促进 VSMC增殖。

AIM: To explore the effects of constant magnetic fields(CMF) of different intensities on the proliferation of human umbilical arterial vascular smooth muscle cells(VSMC). METHODS: The VSMCs of human umbilical arterial were cultured with DMEM containing 100 mL·L -1 neonatal calf serum and fibroblast growth factor(5 μg·L -1 ), epithelial growth factor (10 μg·L -1 ) for 3～10 posterities. 100 μL mixture of DMEM and VSMC(cell density 1×10 5·mL -1 ) were put into the culture plate with 96 holes and incubated for 72 h in the control group which was not under a CMF. The experimental group was first incubated for 24 h, then CMFs with 0.5,1,2,5,10,30,60 mT was put into the culture plate for 48 h. MTT and 3H-TdR incorporation methods (A-value and cpm-value) were used to determine the amount of VSMC proliferation. RESULT: The amount of VSMC proliferation was significantly lower than those of the control group under the CMFs 0.5,1,2,5 mT(P<0.05 ),but was significantly higher than those of the control group under the CMFs 10,30,60 mT (P<0.01 ). CONCLUSION: The CMFs of 0.5,1,2,5 mT can significantly inhibit human VSMC proliferation and CMFs 10,30,60 mT can enhance VSMC proliferation.