RANKL基因在胃癌中的表达及与病理因素的关系

Expression of RANKL in gastric carcinoma and its relationship to pathological parameters

目的 探讨RANKL蛋白在胃癌诊断、转移及预后判断中的作用。方法采用免疫组织化学方法检测 4 5例胃癌组织、2 6例癌旁粘膜组织、10例正常胃粘膜上皮中RANKL蛋白的表达情况。结果 4 5例胃癌组织中RANKL蛋白表达阳性 2 1例 (47% )。在直径≥ 5cm肿瘤、肿瘤穿透浆膜、淋巴结转移阳性以及Ⅱ、Ⅲ、Ⅳ期胃癌中RANKL蛋白的表达显著升高 (P <0 0 5 ) ,2 6例癌旁粘膜中RANKL蛋白呈弱阳性表达 5例 ,正常胃粘膜中RANKL蛋白呈阴性表达。RANKL蛋白在胃癌组织中的表达与在癌旁组织、正常胃粘膜中的表达差异有显著意义 (P <0 0 5 )。结论检测RANKL蛋白表达有助于胃癌的诊断。

ObjectiveTo evaluate the expression of RANKL′s protein for the diagnosis and assessment of tumor metastatic potential and prognosis in patients with gastric carcinoma.MethodsThe expression of RANKL was detected in 45 cases of gastric carcinoma, 26 cases of paracancerous mucosa and 10 cases of healthy control by using ABC immunohistochemical method. Data were tested by χ 2 test for statistical significance.ResultsPositive expression of RANKL protein was detected in 21 out of 45(46 7%) cases. The expression of RANKL significantly increased in tumors ≥5?cm in diameter, with serosa infiltrated, positive lymph node, and in tumors of stage Ⅱ,Ⅲ,Ⅳ( P <0 05). Faint positive expression of RANKL was detected in paracancerous mucosa in 5 out of 26 cases. No expression was detected in the normal gastric mucosa. The difference between gastric carcinoma, paracancerous mucosa, and normal gastric mucosa was all statistically significant( P <0 05).Conclusions Determination of RANKL helps in the establishment of diagnosis and assessment of tumor metastatic potential and prognosis prediction of gastric carcinoma.