部分门静脉动脉化重建肝血流的实验研究

An experimental study on the reconstruction of hepatic blood flow with arterio-portal shunt

目的 了解部分门静脉动脉化重建肝血流对肝脏的影响。方法 建立部分门静脉动脉化重建肝脏血流的大鼠实验模型 ,通过该模型对肝脏的血流量、门静脉压和结构的影响分别进行了为期 1个月和 6个月的观察。结果 大鼠在行部分门静脉动脉化后的肝脏血流量为 (2 5 2± 3 2 )mV、术后 1个月为 (2 3 4± 4 6 )mV与术前 (2 2 6± 2 8)mV相比 ,差异无显著意义 (P >0 0 5 )。术后半年的肝脏血流量 (30 6± 10 8)mV与术前 (2 2 6± 2 8)mV相比 ,差异有显著意义 (P <0 0 5 ) ;术后门静脉压为 (12 9± 1 8)cmH2 O、术后 1个月为 (12 3± 1 4 )cmH2 O与术前 (10 0± 0 4 )cmH2 O相比 ,差异有显著意义 (P <0 0 1) ;术后半年门静脉压 (18 8± 6 3)cmH2 O与术前 (10 0± 0 4 )cmH2 O相比 ,差异亦有显著意义 (P <0 0 1) ;行部分门静脉动脉化后 1个月时肝脏结构无明显变化。术后 6个月时 4只大鼠 (4/ 10 )出现Disse间隙轻度扩张。结论 部分门静脉动脉化术后 6个月可造成较明显的肝脏血流量增加和门静脉压增高 。

ObjectiveTo study the effect of arterio portal shunt(APS) on the liver. Methods Experimental model of APS was established in rats. Operative effect on hepatic blood flow(HBF) and portal venous pressure(PVP) was evaluated and change of hepatic structure was investigated at one month and six months after the operation respectively.ResultsWhile HBF at one month after the operation did not change (compared with normal P >0 05),at six months it changed significantly (compared with normal P <0 05); PVP at one month and six months was higher than normal (all P <0 01). Histological examination revealed no degeneration of the hepatocytes at one month, but slightly Disse's space dilation in 4 of 10 rats at six months was observed.ConclusionsAt six months after the operation APS has changed the HBF and PVP significantly, but APS does not effect negatively on the hepatic structure.