体外肝细胞极性的重建

Reestablishment of cell polarity of hepatocytes in vitro

目的使培养肝细胞在体外重建细胞极性。方法利用三明治构型培养成鼠肝细胞并观察其形态、特异性膜区域蛋白分布并以单层胶原培养肝细胞作对照。结果三明治构型肝细胞呈单层生长 ,形成肝板样结构 ,伴胆小管网络形成 ,免疫组织化学证明肝细胞膜区域蛋白呈特异性分布。而在对照组 ,肝细胞基本没有形成肝板样结构 ,肝细胞膜区域蛋白没有形成特异性分布。结论三明治构型培养肝细胞在体外重建细胞极性 。

Objective To reestablish cell polarity of hepatocytes cultured in vitro.Methods Sandwich configuration was used to culture hepatocytes. The morphology as well as specific plasma proteins were compared with a single collagen configuration.Results In sandwich configuration hepatocytes were arranged in single cell plate, and the gradual development of bile canaliculi like structures and an anastomotic network were observed over the course. Histochemical immunolocation revealed that membrane proteins were distributed specifically. Hepatocytes in single collagen configuration showed hardly any hepatic plate like structure,and the distribution of specific plasma protein was not formed. Conclusions Sandwich cultured hepatocytes reestablished cell polarity structurally and regained the characteristics of hepatocytes in situ.