表皮生长因子对A系小鼠胚腭突细胞增殖代谢的影响

Effects of Epidermal Growth Factors on the Proliferation and Metabolism of A/J Mouse Embryonic Palatal Cells

目的 :研究不同剂量表皮生长因子 (EGF)对A系小鼠胚腭突细胞增殖和DNA、蛋白质及PGE2 合成的影响。方法 :实验分为 9组 ,以平行 4孔 (瓶 )为一组 ,在每组腭突细胞中分别加入EGF使其终浓度为 0 0 0 5ng/ml,0 0 10ng/ml,0 0 5 0ng/ml,0 10 0ng/ml,0 5 0 0ng/ml,1 0 0 0ng/ml,5 0 0 0ng/ml,10 0 0 0ng/ml,5 0 0 0 0ng/ml,每组均设空白对照 ,在加入EGF并培养第 1、3、5天后分别检测细胞的OD值 ,DNA、蛋白质和PGE2 的含量。结果 :EGF可显著增加腭突细胞的OD值 ,促进DNA和PGE2 合成 ,增大了细胞指数。EGF含量在 0 0 0 5～ 10 0 0 0ng/ml浓度范围内 ,呈剂量依赖性 ,10 0 0 0ng/ml时EGF对腭突细胞增殖的作用最强。结论 :EGF显著刺激了A系小鼠胚腭突细胞分裂代谢 ,促进腭突细胞的增殖分化。

Objective: The purpose of this study was to investigate the effects of epidermal growth factors (EGFs) with different concentration on the OD, DNA, protein, and PGE\-2 of A/J mouse embryonic palatal shelves cells (A/J MEPC) isolated from embryonic palatal shelves.Methods: The mouse embryonic palatal shelves cells were grown in different 39 pores (or bottles) with 9 gradient concentrations of EGF (0 005, 0 010, 0 050,0 100, 0 500, 1 000, 5 000, 10 000, 50 000 ng/ml), and four pores were prepared for the same concentration, then the OD, DNA, protein and PGE\-2 of A/J MEPC were measured after 1 day, 3 days and 5 days.Results: EGFs stimulated DNA and PGE\-2 synthesis of A/J MEPC, and augmented proliferation index (PIX). Their effects were very obvious in promoting the proliferation of A/J MEPC, when the concentration was 10 000 ng/ml.Conclusion: EGF may be important in regulating proliferation and metabolism of embryonic palatal shelves cells.\;