TNP-470作用于体外培养GNM细胞系的形态学研究

Effect of Angiogenesis Inhibitor (TNP-470) on the Morphology of GNM Cell Line in vitro

目的 :观察TNP_4 70作用于体外培养的人口腔鳞癌颈淋巴结转移癌细胞系GNM后的形态学改变 ,探讨TNP_4 70的作用机理。方法 :利用体外培养技术 ,采用相差显微镜、扫描电镜、透射电镜观察法 ,观察TNP_4 70对GNM细胞形态及超微结构的影响。结果 :TNP_4 70 (2 μg/ml)作用于GNM细胞 4 8h ,大量细胞变圆、细胞膜皱缩 ,并出现较多悬浮死亡细胞。作用 72h后 ,扫描电镜下可见细胞表面的微绒毛数量减少、长度变小、细胞一端有较大的球状突 ;透射电镜下可见GNM细胞大量坏死、线粒体和内质网破坏。结论 :TNP_4 70对GNM细胞具有明显杀伤作用 ;TNP_4 70可使GNM细胞线粒体和内质网发生破坏 ,这可能是其具有直接癌细胞毒作用的机制之一 ;TNP_4

Objective: The purpose of this study was to investigate the effect of an angiogenesis inhibitor (TNP_470) on the ultra micro_structural morphological changes of GNM cell line, which was derived from human oral squamous cell carcinomas in vitro.Methods: The GNM cells were cultured and, the effect of TNP_470 on ultra micro_structural morphological changes of GNM cells was observed under the inverted microscope, the scanning electron microscope (SEM) and the transmission electron microscope (TEM).Results: Numerous round cells, shrinkage of cellular membrane and dead cells were observed 48 hours after 2 μg/ml of TNP_470 was added into the GNM cellular suspension. After 72 hours, GNM cells became shortened and, the number of microvilli of the cellular surface was observed under the SEM and TEM. A large number of GNM cells turned into necrosis, accompanying with the destruction of mitochondria and endoplasmic reticula.Conclusion: TNP_470 has a strong tumor cytotoxic effect on GNM cells, which may be due to its destructibility on mitochondria and endoplasmic reticula of GNM cells. TNP_470 can alter the surface structure of GNM cell membrane, which suggests that TNP_470 may interrupt the metastasis of GNM cells.\;