口腔颌面部非何杰金氏淋巴瘤流式细胞DNA分析及意义探讨

Flowcytometry DNA Analysis of Oral and Maxillofacial Non-Hodgkin′s Lymphoma

目的 :探讨流式细胞学各项指标与非何杰金氏淋巴瘤 (NHL)发病部位、临床分期、病理分级、细胞类型及预后的关系和流式细胞学对NHL的诊断价值。方法 :采用流式细胞仪测定 5 0例口腔颌面部NHL及 10例反应性增生淋巴结 (RLN)石蜡包埋组织的DNA倍体、细胞周期各参数 ,并分析各参数及其与预后的关系。结果 :10例RLN皆为二倍体 ,而NHL二倍体率为 5 4 % ,异倍体率为 4 6 % ,二者有显著性差异。SPF值、S +G2 /M值与非何杰金氏淋巴瘤恶性程度密切相关 ,SPF值、S +G2 /M值随肿瘤的恶性程度增大而增大。结论 :口腔颌面部淋巴瘤流式细胞学检测具有一定诊断学价值 ,但其运用一定要与形态学相结合。同时SPF、S +G2

Objective: The purpose of this study was to investigate the relationship between the results of flowcytometry analyses of different clinical stage, location, pathologic grade and cell origin of oral and maxillofacial non_Hodgkin′s lymphoma (NHL), and the diagnostic value of flowcytometry analysis in lymphoma.Method: This study analyzed 50 oral and maxillofacial NHL cases and 10 reactive lymph nodes (formalin fixed and paraffin embedded) by flowcytometry (FCM).Results: Reactive lymph nodes were all diploid. The diploid rate of NHL was 54%, and aneuploidy rate was 46%. There was statistically significant difference between reactive lymph nodes and NHL in the DNA ploidy status and cell cycle data (SPF, CV, S+G2/M, DI). The S phase fraction (SPF) and S+G2/M had close relationship with the grade of NHL. SPF value and DNA ploidy status had no obvious relationship with the prognosis.Conclusion: The results suggested that the FCM had diagnostic value in NHL, especially when the morphological diagnosis was difficult. Although the cell cycle data had no prognostic value, SPF and SPF+G2/M can show the proliferative status of NHL, which can help clinical doctor select therapeutic method.\;