变应性鼻炎基因芯片检测及其基因表达谱的研究

Gene chip screen in allergic rhinitis and preliminary analysis of the differential expressed genes

目的 运用基因芯片技术研究变应性鼻炎的基因表达谱。方法 对变应性鼻炎和非变应性鼻炎鼻黏膜组织进行总RNA抽提 ,纯化后的mRNA进行逆转录制备杂交探针 ,应用含有 12 80 0个人类全长互补DNA(complementaryDNA ,cDNA)的表达谱芯片对 8例变应性鼻炎与非变应性鼻炎鼻黏膜组织进行差异表达谱分析。结果 在变应性鼻炎的基因表达谱中差异表达基因共有 734条 ,上调的基因 4 30条 ,下调的基因 30 4条 ,共存性差异表达基因有 2 1条 ,其中上调基因 15条 ,下调基因 6条 ,在差异表达的基因中 ,其中有些是炎性反应、免疫应答、免疫调控、神经内分泌网络系统及信号传导的相关基因 ,这些基因可能与变应性鼻炎发病机制有关。结论 运用基因芯片技术研究变应性鼻炎相关基因 。

Objective To discover the etiology of allergic rhinitis(AR) at the genomic level Methods Microarray analysis on a genomic scale was used to find changes in genes expression in AR The total RNAs were isolated from nasal mucosa of AR and normal, then purified to mRNAs and were reversely transcribed to cDNAs with the incorporation of fluorescent labeled dUTP to prepare for the hybridization probes The genes differential expressed in AR were screened with the gene chip which contained 12 800 human genes in all 8 samples Result 734 genes were shown in differential expressed profile with 430 upregulated genes and 304 downregulated genes, and all 8 samples were 15 genes upregulated and 6 genes downregulated The differential expressed genes involved in the functions of inflammation, immunology, neuroendocrine and signal conduct, and they had potential value in AR study Conclusion The investigation based on gene chip in researching related genes in AR afforded a new idea in studying pathogenesis of nasal allergic diseases