格林-巴利综合征与人类白细胞抗原关联的研究

The association between HLA typing and different subtypes of Guillain-Barré syndrome

目的 研究格林 巴利综合征 (GBS)两种不同亚型 (AIDP和AMAN)与HLA Ⅰ类和Ⅱ类等位基因分型的关系 ,探讨AIDP和AMAN患者免疫遗传的特点。方法 用改良快速盐析法抽提基因组DNA ,采用聚合酶链反应 顺序特异性引物法 (PCR SSP)对 6 4例AIDP、AMAN患者和 132例健康人进行HLA Ⅰ类和Ⅱ类等位基因分型。结果 AIDP组HLA Ⅰ类基因中A33的频率较对照组升高 ,相对风险率 (RR)为 6 13,校正的P值 (Pc)为 0 0 11(P值 <0 0 5 )。HLA Ⅱ类基因中 ,DR16和DQ5的频率较对照组升高 ,RR值分别为 8 2 8和 3 4 7,Pc分别为 0 0 14和 0 0 2 5 (P值均 <0 0 5 ) ;AMAN组HLA Ⅰ类基因中B15、B35频率较对照组升高 ,RR值分别为 4 0 9和 7 0 8,Pc分别为 0 0 15和 0 0 0 0 8(P值均<0 0 5 )。结论 HLA A33、DR16和DQ5与AIDP的易感性可能有关。HLA B15、B35与AMAN的易感性可能有关。未发现HLA

Objective To study the relationship between the susceptibility to two subtypes of Guillain Barre syndrome(GBS) AIDP and AMAN and the frequency of HLA class Ⅰ and Ⅱ alleles as well as to approach the characteristics of AIDP and AMAN patients in immune genetics Methods A case control research was done on 31 AIDP and 33 AMAN and 132 health individuals DNA was extracted from peripheral blood leucocytes by improved fast salting out HLA genes were typed with DNA based technology and PCR sequence specific primers (PCR SSP) method Results HLA A33 frequency of HLA class Ⅰ, and DR16 and DQ5 frequencies of HLA class Ⅱ showed a significant increase in AIDP group as compared with the control Relative risk ( RR ) was 6 13, 8 28 and 3 47 respectively Corrected probability ( Pc ) was 0 011, 0 014 and 0 025( P <0 05) HLA B15,B35 frequency of HLA class Ⅰshowed a significant increase in patients with AMAN as compared with the controls, RR was 4 09 and 7 08 respectively, Pc was 0 015 and 0 000 8 respectively Conclusions HLA A33, DR15 and DQ5 may have association with susceptibility to AIDP; HLA B15, B35 may have association with susceptibility to AMAN HLA classⅡgenes were not found to have any association with AMAN