口蹄疫病毒抗原表位的编码序列在大肠杆菌中表达

The Expression of the Coding Sequence for Foot-and-Mouth Disease Virus′s Antigen Epitope in Escherichia coli

为在大肠杆菌和牛分枝杆菌 BCG中表达口蹄疫病毒 ( Foot-and-Mouth Disease Virus,FMDV) O、A、C三种血清型抗原表位的编码序列 ,我们优化筛选了这三种血清型主要抗原表位的序列 ,设计合成了其编码 DNA序列 ,并将此序列克隆到细菌表达载体 p QE4 1中 ,转化大肠杆菌 M1 5 [p REP4 ],经 IPTG诱导 ,在 SDS-PAGE中得到 34 k D的小鼠二氢叶酸还原酶( DHFR)基因与 FMDV的三型抗原表位编码序列的融合表达的蛋白质带 .

The antigen epitope′s coding sequences of O, A, and C three serotypes of the Foot and Mouth Disease Virus (FMDV) were designed and synthesized, and the sequence was cloned into bacterial expression vector pQE41, named pQFM01, and was transformed into E. coli M15 , after induced by IPTG, a 34kD protein band, which is the fusion protein of mouse DHFR and FMDV antigen epitopes, was shown on SDS PAGE.