大蹼铃蟾蛋白提取物对膀胱癌细胞株的抑制作用及对端粒酶的影响

Effects of bombina maxima protein on growth of bldder cancer cell lines and expession of telomerase

目的 探讨大蹼铃蟾蛋白提取物对膀胱癌细胞株的抑制作用及对端粒酶的影响。方法 采用肿瘤细胞培养方法 ,检测大蹼铃蟾蛋白提取物对膀胱癌细胞株T2 4、BIU 87、ScaBer的抑制作用 ;以TRAP PCR ELISA方法检测大蹼铃蟾蛋白提取物对膀胱癌细胞株端粒酶表达的影响。结果 大蹼铃蟾蛋白提取物表现出对膀胱癌细胞株有较强的抑制作用 ;对膀胱癌细胞株端粒酶表达则无影响。结论 大蹼铃蟾蛋白提取物对多个膀胱癌细胞株具有直接细胞杀伤作用 。

AIM To study the boosting effect of Bombina maxima protein on bladder cancer cell lines growth and expression of telomerase. METHODS Cell culture was used to dectect the effects of 3 bladder cancer cell lines growth;TRAP PCR ELISA method was used to detect the telomerase express under the ID 50 culture concentration. RESULTS Bombina maxima protein had the strong effect on growth of cell lines;particularly in the first 48 h; no effect was found in telomerase expression. CONCLUSION Bombina maxima protein has the direct inhibitory effect on bladder cancer cell lines growth;The mechanism of antitumor of Bombina maxima protein is not by telomerase.