重组人CTLA4Ig融合蛋白体外对人T淋巴细胞功能的影响

Effects of rhCTLA4Ig on human T lymphocyte in vitro

目的 研究重组人CTIA4Ig对人T淋巴细胞功能的影响。方法 采用初次混合淋巴细胞培养、再次混合淋巴细胞培养及细胞毒实验的方法。结果 CTLA4Ig在浓度高于3mg·L-1时能有效地抑制初次混合淋巴细胞反应 (P <0 0 5 ) ,其在作用 2 4h内不能发挥有效的抑制作用 ,72h能达最大抑制效率 ;在再次混合淋巴细胞反应实验中CT LA4Ig作用后的T淋巴细胞对同一供者的外周血单核细胞的刺激表现出特异性无反应 ,而对无关供者的外周血单核细胞的刺激能产生正常的应答反应。结论 CTLA4Ig在体外能抑制T细胞的增殖 。

AIM To study the effect of rhCTLAIg on human T lymphocytes. METHODS Primary mixed lymphocyte reaction (MLR), secondary MLR and cytotoxicity assay were used. RESULTS CTLA4Ig significantly inhibited primary MLR( P< 0 05) and the maximal inhibition was achieved at 72 h of incubation with CTLA4Ig. Secondary MLR experiment showed cells primed in the presence of CTLA4Ig had a specific hyporesponsiveness when challenged with PBMC from the original donor, yet responded normally to PBMC from the third party donor. CONCLUSIONS CTLA4Ig can inhibit T cell proliferation and induce T cell anergy in vitro by blocking B7/CD28 co stimulatory pathway.