免疫荧光法检测L-NNA对脑缺血再灌流后海马区GFAP合成的影响

Effect of L-NNA on GFAP production in the gerbil hippocampus after recirculation following ischemia

目的 探讨NG 硝基 L 精氨酸 (L NNA)对沙土鼠海马区胶质纤维酸性蛋白 (GFAP)合成的影响。方法 钳夹沙土鼠的双侧颈总动脉制造脑缺血模型 ,应用免疫荧光法染色。结果 脑缺血再灌流后海马区GFAP合成增加 ,GFAP阳性细胞主要分布在放射层及分子层 ,L NNA能抑制海马区GFAP的合成。结论 L NNA是一氧化氮合酶强的抑制剂 ,L

AIM To observe the effects of L NNA on glial fibrillary acidic protein (GFAP) production in the gerbil hippocampus after recirculation following ischemia. METHODS Both vessels occlusion and immunofluorescent methods in gerbil hippocampal tissue slices incubated in vitro were used. RESULTS Recirculation following ischemia led to a rise on GFAP concentration in the hippocampus. GFAP positive cells mainly distributed in rediatum layer and molecullar layer in the hippocampus, L NNA decreased GFAP synthesis. CONCLUSION L NNA is a strong inhibitor of nitric oxide synthase(NOS). It is possible that the role of endogenous NO mediated GFAP synthesis is inhibited by L NNA.