同种异体神经移植中环孢素A的最短有效时间

Shortest Effective Duration of Cyclosporin A Immunosuppressive Therapy for Allogenic Nerve Graft

目的 :研究同种异体神经移植中环孢素A(cyclosporinA ,CSA)的最短有效时间。方法 :72只SD鼠分为A组 ,自体原位移植 ;B组 ,异体移植应用CSA免疫抑制 3周 ;C组 ,异体移植应用CSA免疫抑制 4周 ;D组 ,异体移植应用CSA免疫抑制 5周 ;E组 ,异体移植应用CSA免疫抑制 6周 ;F组 ,异体移植无免疫抑制。各组分别于术后 6周和 12周对实验动物的功能学指标 (步态分析 )、电生理学指标 (潜伏期、运动神经传导速度 )、形态学指标 (腓肠肌湿重 ,坐骨神经光镜、电镜观察 )进行检测。结果 :6周时各组实验动物神经再生不完全 ,功能未恢复。 12周时A ,D ,E组动物在功能学指标、电生理指标及光镜、电镜指标上均优于其它各组。而A ,D ,E组间在上述各指标上无显著性差异。结论 1.0cm缺损的异体神经移植动物实验中 ,应用CSA 5mg/ (kg·d)

Objective: To determine shortest effective time of cyclosporin A treatment required to induce adequate immunosuppression after allogenic nerve graft in rats model. Methods: This study was carried out on 72 male SD rats randomly divided into 6 groups of 12 animals each. ① Group A, autograft; ②Group B, allograft with CSA for 3 weeks; ③ Group C, allograft with CSA for 4 weeks; ④ Group D, allograft with CSA for 5 weeks; ⑤ Group E, allograft with CSA for 6 weeks; ⑥ Group F, allograft with no treatment. All operative procedures and measurements of parameters were performed by one operator. Functional test (track analysis ), electrophysiological parameters, morphological parameters ( weight of gastrocnemius muscle, light and electron microscopy of sciatic nerve ) were analyzed at 6 weeks and 12 weeks post operatively. Results: At 6 weeks, there was no functional recovery and sciatic nerve regeneration was incomplete in all groups. At 12 weeks, animals from Group A, D and E showed better functional , physiologic, electron and light microscopy findings (results) than animals from any other groups. There was no significant difference in the results between Group A, D and E. Conclusions: Using CSA 5mg/(kg·d) for 5 weeks is the minimum effective treatment course for peripheral nerve allograft in rats models.