肝细胞肝癌组织中P27蛋白的表达及其意义

The Significance of P27 Expression in Hepatocellular Carcinoma

目的:探讨P27蛋白在肝细胞肝癌组织中的表达及其与临床病理学特征的关系。方法:采用免疫组织化学S-P染色法检测33例肝细胞癌组织中P27蛋白表达;;并对阳性颗粒进行图像分析。结果:P27平均吸光度值(A)和肝细胞肝癌的恶性程度密切相关;;在高分化、无癌栓、直径<3cm的肝癌组织中;;平均A值明显高于中低分化、有癌栓直径>3cm的组织。结论:P27蛋白表达量的减少和肝细胞肝癌的恶性程度及侵袭性密切相关;;可作为一个新的预后指标。

Objective: To investigate the expression of P27 protein in hepatocell ular carcinoma and its relationship with clinic pathology. Methods:P27 protein was measured by immunohistochemistry method(S-P method)and the positive cells o f P27 were analyzed by computer image analysis system. Results:The mean optical density of P27 is significantly connected with the malignant grade of hepatocel lular carcinoma. The mean optical density of P27 in the hepatocellular carcinoma which well differentiated,had no cancerous embolus and was less than 3 cm in d iameter was significantly highly than in the hepatocellular carcinoma which diff erentiated worse,had cancerous embolus and was above 3 cm in diameter.Conclusio ns:The decreased expression of P27 is strikingly related to malignacy and biolo gical aggressiveness in hepatocellular carcinoma,which may serve as one of a ne w prognosis factor in hepatocellular carcinoma.