摘要
目的:观察中华眼镜蛇毒Ｆ组分对血小板α－颗粒膜蛋白表达的影响,确定Ｆ组分是否具有抑制血小板活化的功能。方法:实验分成对照组、凝血酶组和各给药组(凝血酶＋Ｆ组分),应用酶联免疫吸附法(ＥＬＩＳＡ)测定健康成年人血浆中的α－颗粒膜蛋白(ＧＭＰ－１４０)含量的变化。结果:凝血酶能引起血浆中的ＧＭＰ－１４０含量明显升高,中华眼镜蛇毒Ｆ组分 １００、３０ μｇ/ｍＬ明显降低凝血酶引起的血浆ＧＭＰ－１４０含量升高,并呈现一定程度的剂量依赖关系,两组比较Ｐ＜０．０５。结论:中华眼镜蛇毒Ｆ组分能够抑制激动剂引起的血小板活化。
To study the effect of fraction F of Naja Naja atra venom on expression of alpha granule membrane protein( GMP140) of platelet,and to determine whether the fraction F can inhibit the activation of platelet. Methods: three groups were included in the study: control group, thrombin group and treatment group ( thrombin + fraction F). The content of GMP140 in plasma was determined by enzyme-linked immunosorbent assay ( ELISA ). Results: Thrombin significantly increased the level of GMP140 in plasma, and the fraction F of Naja Naja atra venom remarkably inhibited the production of GMP140 induced by thrombin at dosages of 100, 30 and 10 μg/mL respectively, with a manner of dose-dependent. Significant difference was observed between two groups (P < 0. 05 ). Conclusion: Fraction F of Naja Naja atra venom can inhibit the activation of platelet induced by angonist.
出处
《广州医学院学报》
2002年第1期1-3,共3页
Academic Journal of Guangzhou Medical College
基金
广东省自然科学基金(980463)
卫生部科学基金(98-2-349)
广东省医学科技基金(A1999223)项目
关键词
中华眼镜蛇毒F组分
血小板
Α-颗粒膜蛋白
酶联免疫吸附法
活化
fraction F of Naja Naja atra venom
platelet
alpha granule membrane protein
activation
enzyme-linked immunosorbent assay
