CEA-rV对巨噬细胞抗原提呈功能的影响

The Effect of CEA-rV on Antigen-presenting Ability of Macrophage

目的:探讨 ＣＥＡ－ｒＶ对巨噬细胞抗原提呈功能的影响。方法:采用混合淋巴细胞反应(ＭＬＲ)检测 Ｍφ的抗原提呈功能,采用流式细胞仪(ＦＣＭ)检测Ｍφ表面分子ＭＨＣ－Ⅰ(Ｈ－２Ｋｂ)、ＭＨＣ－Ⅱ(Ⅰ－Ａｂ)及Ｂ７(Ｂ７－２)的表达,采用荧光抗体直接染色法检测肿瘤细胞表面Ｈ－２Ｋｂ、Ⅰ－Ａｂ的表达。结果:ＣＥＡ－ｒＶ接种小鼠腹腔Ｍφ组(ＣＥＡ－ｒＶＭφ组)Ｔ细胞分泌ＩＬ－２的水平较Ｗ－ＶＶ及ＶＳ组显著增强(Ｐ＜０．０１),小鼠腹腔Ｍφ自身表达的Ⅰ－Ａｂ和Ｂ７－２分子较低(分别为４１．７%,１３．７%),接种Ｗ－ＶＶ后其Ⅰ－Ａｂ及Ｂ７－２分子表达改变不明显(分别为４４．３%,１５．ｌ%),而接种 ＣＥＡ－ｒＶ后其Ⅰ－Ａｂ及 Ｂ７－２分子表达明显增强(分别为 ８７．２%,３９．５%)。各组ＣＥＡ阳性肿瘤细胞表面分子ＭＨＣ－Ⅰ强表达,却没有 ＭＨＣ－Ⅱ表达。结论:ＣＥＡ－ｒＶ腹腔接种 Ｍφ可使 Ｍφ抗原提呈功能显著增强,Ｍφ的表面分子ＭＨＣ－Ⅱ与Ｂ７－２表达增强可能是其抗原提呈功能增强的主要原因。提示ＭＨＣ－Ⅱ与Ｂ７－２可能在ＣＥＡ－ｒＶ抗瘤中发挥重要作用。

Objective: To investigate the effect of CEA-rV on antigen-presenting ability of macrophage( Mφ). Methods :In this study, the antigen-presenting ability of Mφ was detected by mix lymphocyte reaction ( MLR) , the expressions of cell surface molecules MHC-Ⅰ(H-2Kb)、MHC-Ⅱ(I-Ab) and B7(B7-2) on mouse Mφ were detected by flow-cytometry (FCM),and the expressions of H-2Kb and I-Ab on CEA positive tumor cell surface were detected by direct fluorescence antibody staining. Results: IL-2 level from T cells incubated with CEA-rV Mφ was significantly increased compared to that of W-VV group or NS group ( P < 0. 01 ). The expressions of I-Ab and B7-2 on mouse Mφ.treated with CEA-rV were significantly increased (87.2% and 39.5 % respectively) compared to that on W-VV treated Mφ(44.3% and 15. 1% respectively) or Hanks treated Mφ(41.7% and 13.7% respectively) ,while there was no significant difference in the expression of H-2K between different groups. Strong MHC-Iexpression, but not MHC-Ⅱ expression on the CEA positive tumor cellswere revealed by routine fluorescence-activated immunohistochemistry analysis,and there was no difference between the CEA-rV treated group.Hanks treated group and W-VV treated group. Conclusion: It could be deduced that CEA-rV enhances the antigen-presenting ability of CEA-rV Mφ which may due to the upregulaion of the expression of I-Ab.B7-2 on CEA-rV Mφ. I-Ab and B7-2 may play important roles in anti-tumor of CEA-rV.