川芎嗪对大鼠视网膜缺血后细胞凋亡的影响

Effect of Tetramethylpyrazine on Apoptosis of Retinal Cells after Retinal Ischemia in Rats

目的:观察中药制剂川芎嗪对ＳＤ大鼠视网膜缺血后细胞凋亡的影响。方法:采用前房灌注生理盐水的方法做成视网膜缺血模型,治疗组每天腹腔注射川芎嗪１次(５０ ｍｇ/ｋｇ)。Ｔｕｎｅｌ法检测各组视网膜凋亡细胞,结果:缺血组凋亡细胞明显高于川芎嗪治疗组,同一时间段各组之间相比差异均有显著性。结论:川芎嗪可减少缺血后视网膜细胞的凋亡,具有明显的保护作用。

Objective:To investigate the effect of Tetramethylpyrazine on apoptosis after transient retinal ischemia. Methods: A model of retinal ischemia was established by injecting saline into the anterior chamber of SD rats. Tetramethylpyrazine was given peritoneally in the treatment group once daily. The retinal apoptosis cells were detected by terminal - deoxynucleotidyl transferase mediated nick end labeling ( Tunel) . Results: The number of apoptosis cells in ischemia group were significantly higher than that of the treatment group, there was significant difference between two groups at each time-point. ConclusionTetramethylpyrazine has the protective effect against apoptosis in ischemic retina.