云南稻种抗病基因同源序列类似性分析

Analysis of Resistance Gene Analogue for Rice Cultivars in Yunnan Province

利用已知植物抗病基因和蛋白激酶序列中的保守区域设计的 3对引物对 137个水稻品种DNA进行PCR扩增及聚丙烯酰胺凝胶电泳分析。结果表明 ,水稻抗病基因同源序列类型丰富 ,以带型差异类似性 96 %为标准 ,这些品种可划分出籼粳差异较为明显的 3个类群 ;以带型差异类似性 6 0 %为标准 ,这些品种可划分为 2 0个类群。这些依分子标记划分的类群与品种的亲缘关系和抗瘟性基本一致。

Genetic diversity of commercial and local rice cultivars in Yunnan Province was studied using resistance gene analogue (RGA) based on resistance gene conserved sequences. The RGA analysis of 137 cultivars was conducted by PCR amplification using three primers, i.e. S1/AS3, XLRR for/ XLRR rev, and Pto kin1/Pto kin2, respectively. The results showed that both Indica and Japonica cultivars were genetically highly diverse. All cultivars were divided into 3 lineages according to the DNA band data at 96% dissimilarity, and into 20 lineages at 60% dissimilarity. The lineages were related to their genetic background and blast disease resistance with only a few exceptions. The RGA data can be useful in rice production by mixed planting of different cultivars in the field and breeding of resistance cultivars by selecting different parental cultivars with great gene tic diversity.