外源Bt杀虫蛋白和棉花主要抗虫萜烯类物质互作关系研究

Study on the Interactions Between Exogenous Bt-ICP and Cotton Terpenoids Chemicals

利用高效液相色谱 (HPLC)技术比较了不同生长时期转Bt基因棉花及其对照亲本不同器官中抗虫萜烯类物质含量的差异。显著性分析发现 ,外源Bt杀虫蛋白表达对棉花原有的棉酚、总杀实夜蛾素及总抗虫萜烯类合成量不存在不利的影响。以棉酚作为萜烯类物质代表物 ,在饲料中进行了棉铃虫幼虫对棉酚和Bt杀虫蛋白的复合剂量反应试验。结果表明 ,棉酚对棉铃虫幼虫的抗虫作用达到极显著水平 ,棉酚和Bt杀虫蛋白之间没有交互作用 。

The contents of terpenoids chemicals in Bt transgenic cotton and their parental varieties were analyzed by HPLC method. Statistical analysis of variance showed that Bt ICP expression has no negative effect on synthesis of gossypol, total heliocide and total resistant terpenoids. The results of the combined dosage test of Bt ICP and gossypol in vitro showed that there was no interaction between gossypol and Bt ICP on the mortality of cotton bollworm larvae. This means the actions of Bt ICP and gossypol on cotton bollworm are additive. Therefore, it is advantageous to combine Bt ICP with cotton terpenoids chemicals in controlling cotton bollworm.