鸡源大肠杆菌1型菌毛菌株粘附鸡呼吸道上皮的电镜观察

Observation with Electron-microscope on Adherence of Type 1 Pili in Avian Strain of Escherichia coli to Chicken Tracheal Epithelium

用电子显微镜观察了鸡大肠杆菌 (O78) 1型菌毛在不同温度中的表达情况。细菌在营养肉汤中 37℃培养 4 8h可表达大量的菌毛 ,而 18℃培养 4 8h则缺乏菌毛。扫描电镜观察结果 ,37℃培养的鸡大肠杆菌可大量粘附鸡呼吸道上皮粘膜 ,而 18℃培养的同种菌则不能粘附 ,表明鸡大肠杆菌粘附鸡呼吸道上皮的过程由

An avian Escherichia coli (O 78 ) strain was observed for type 1 pili expression with electron microscope.A great deal of type 1 pili was produced after avian strain of Escherichia coli was cultured in broth at 37 ℃ for 48 h,while little pili was produced after the strain was cultured at 18 ℃ for 48 h.The adherence of Escherichia coli cultured in in broth at 37 ℃ to chicken tracheal epithelium cells was visualized by use of scanning electron microscope,but the bacterium cultured at 18 ℃ lacked this effect.It proves that adherence of Escherichia coli to chicken tracheal epithelium is mediated by type 1 pili.