浙江水稻稻种病原细菌多样性研究

DIVERSITY OF PATHOGENIC BACTERIA FROM RICE SEEDS

1997～ 2 0 0 0年间对以浙江平原、丘陵及山区为主的稻种病原细菌开展了种类、频率及传病率的研究。从 30 3份稻种样本中分离出 390 6个菌株 ,经菌落形态、细菌学、致病性测定后获得 2 0 1个致病或弱致病菌 (占总数的 5 .15 % )。选出致病及非致病代表菌株 2 99个连同 2 3个对照菌株用 Biolog进行证实。结果表明种子上所带的病原细菌涉及到 6个致病细菌种和 5个弱致病种。平原稻区存在较多致病细菌种 ,山区较少 ,但两地均以弱致病细菌种占优势 ;丘陵的强致病细菌种的分离频率显著高于平原与山区。在稻种上携带的 11个细菌种中 ,人工接种和自然感染的病稻种均能引起传病的有 Xan-thomonas oryzae pv.oryzicola,Pseudomonas fuscovaginae及 Acidovorax avenae subsp.avenae.

A survey on diversity of pathogenic bacteria including frequency and transmission of the pathogens from plain, hill and mountain areas of Zhejiang Province was conducted during 1997-2000. More than 3 906 bacterial isolates were recovered from 303 rice seed samples. Two hundreds and one pathogenic bacteria accounting for 5.15% of the total isolates were obtained after colony morphology, bacteriology and pathogenicity test. Together with 23 standard reference strains 299 pathogenic and representative nonpathogenic isolates were confirmed by Biolog. Results showed that the seedborne bacteria belonged to 6 higher virulent and 5 low virulent species. The pathogenic species isolated from the samples of plain area were more than those from mountain area. However, the low virulent species were prevalence in the two areas. Isolation frequency of the higher virulent species from hill was significantly higher than those from other areas. Among 11 seedborne bacteria only Xanthomonas oryzae pv. oryzicola, Pseudomonas fuscovaginae and Acidovorax avenae subsp. avenae were confirmed as seedborne and seed transmission by using both artificial inoculated and naturally infected seeds.