粉虱传双生病毒的TAS-ELISA及PCR快速检测

RAPID DETECTION OF WHITEFLY-TRANSMITTED GEMINIVIRUSES BY TAS-ELISA AND PCR

利用粉虱传双生病毒 (WTGs)多克隆抗体及单克隆抗体 ,建立了三抗体夹心 EL ISA (TAS-EL ISA)检测 WTGs的方法 ,并发现了单克隆抗体 SCR18可广泛用于我国 WTGs的检测。利用根据WTGs基因组上基因间隔区及外壳蛋白基因保守序列设计的引物 ,建立了 PCR特异检测 WTGs的方法。对田间病样的 TAS- EL ISA和 PCR检测表明 ,粉虱传双生病毒在云南省烟草、番茄和南瓜上均存在 ,2种方法的测定结果是一致的。

Triple antibody sandwich ELISA (TAS ELISA) was established to detect whitefly transmitted geminiviruses (WTGs) with polyclonal and monoclonal antibodies (MAb) that were raised against WTG. The tests showed that MAb SCR18 could be widely used for detection of WTGs in China. The PCR method was also established to detect WTGs with primers PA and PB, which were designed based on the conserved sequences in intergenic region and coat protein gene on WTGs genomes. Field samples were tested with TAS ELISA and PCR method.The result showed that WTGs exist on tobacco, tomato and squash in Yunnan Province and the result of TAS ELISA detection was consistent with PCR result.