核黄素/银溶胶体系的吸收光谱和表面增强共振喇曼光谱

ABSORPTION AND SURFACE ENHANCED RESONANT RAMAN SPECTRA OF RIBOFLAVIN IN SILVER COLLOID

利用可见紫外吸收光谱和表面增强共振喇曼散射光谱(SERRS)研究了核黄素和银胶颗粒的相互作用。不仅有效地淬灭了黄素发色团的荧光干扰,而且还使其喇曼散射得到显著增强。对SERRS进行了分析和指认,SERRS结果揭示出,黄素发色团与银胶颗粒相互作用所形成的络合物,不同于与银离子所形成的络合物。利用这一方法还得到1000cm^(-1)以下的喇曼带,这些带多与发色团的环呼吸振动和C—H面外变型振动有关。

The interaction between riboflavin and silver colloidal particles was studied with absorption and surface enhanced resonant Raman spectroscopy (SERRS). It was found that flourescence interference was completely quenched while Raman scattering was significantly enhanced upon addition of silver sol into ri boflavin aqueous solution. Tentative assignment of Raman modes was given. SERRS sp ectra suggest that Ag complexation of riboflavin in silver sol dose not resemble the aqueous solution complex of Ag. In addition, Raman bands below 1000 cm-1, which are mostly related to ring breathing and C-H torsion out of plane, were also obtained.