支气管哮喘患者粘附分子和细胞因子的变化及其意义

The change and significance of adhesion molecules and cytokine inperiheral blood with bronchial asthma patients

目的:探讨支气管哮喘患者外周血白细胞粘附分子β2整合素(β2-integrin熏CD18)、血清可溶性细胞间粘附分子-1(sICAM-1,CD54)、白细胞介素-6(IL-6)和白细胞介素-8(IL-8)的变化及其意义。方法:采用流式细胞仪技术检测外周血白细胞CD18和血清sICAM-1的表达;采用酶连接免疫吸附方法(ELISA)检测血清IL-6和IL-8水平。结果:(1)与正常对照组相比,支气管哮喘患者外周血白细胞CD18和血清sICAM-1表达显著增加穴P<0.05雪;(2)与正常对照组相比,支气管哮喘患者血清IL-6和IL-8水平显著增加穴P<0.05雪;(3)支气管哮喘患者外周血白细胞CD18和血清sICAM-1表达呈显著正相关(r=0.791熏P<0.05)。结论:支气管哮喘患者外周血白细胞CD18和血清sICAM-1表达以及IL-6和IL-8水平增加,可能是支气管哮喘重要的发病机制之一。

Objective: To investigate the change and significance of adhesion molecules and cytokine in periheral blood with bronchial asthma patients. Method: The expression of CD18 and sICAM-1 were determined using flow cytometry. The level of IL-6 and IL-8 were analyzed using enzyme-linked immunosorbent assay(ELISA). Result: (1)The expression of CD18 and the level of sICAM-1 were significant increased compare with normal control subjects (P<0.05). (2)The level of IL-6 and IL-8 were significantly increased compare with normal control subjects.(3)The positive correlation was found between CD18 and sICAM-1 in bronchial asthma patients(r = 0.791,P<0.05). Conclusion:The increase of CD18, sICAM-1, IL-6 and IL-8 may play an important role in the pathogenesis of bronchial asthma.