摘要
目的 :探讨睡眠剥夺对大鼠脑组织一氧化氮 (NO)及一氧化氮合酶 (NOS)影响。方法 :采用小平台水环境法 (FlowerPot)制作大鼠睡眠剥夺模型 ,采用化学法和酶法观察不同时间睡眠剥夺后大鼠额叶、海马、中脑和下丘脑NO含量及NOS活性变化。结果 :与正常对照组及大平台组比较 ,大鼠在SD后额叶和海马的NO含量及NOS活性增高 ,有显著性差异 (P <0 .0 1~ 0 .0 5 ) ,其余脑区无显著性差异 (P >0 .0 5 )。随着剥夺时间的延长 ,额叶和海马NO含量及NOS活性增高更加明显。结论 :睡眠剥夺可致NO及NOS升高 ,可能与其学习障碍有关 。
Objective: To explore the brain levels of nitric oxide (NO) and nitric oxide synthase(NOS) in rats after sleep deprivation. Methods: NO levels and NOS activity were assayed in different brain regions of Sprague-Dawley rats after sleep deprivation by flower pot compare with normal group (CC) and tank control groups (TC). Results: NO levels and NOS activity were increased in front cortex and hippocampus after SD compared with CC and TC (P<0.01~0.05). Conclusion: The NO levels and activity of NOS are increased after sleep deprivation, NOS/NO may play an important role in learning and memory and modulating sleep
出处
《中国临床心理学杂志》
CSCD
2002年第2期106-107,125,共3页
Chinese Journal of Clinical Psychology