自身免疫性疾病患者可提取核抗体谱与体液免疫的关系

ANTI-ENA AUTOANTIBODY,PROFILES AND HUMORAL IMMUNE ACTIVATION

目的 :探讨可提取核抗原 (ENA)抗体谱的临床意义及其与其他免疫指标的关系。方法 :对 1 1 7份自身免疫性疾病患者血清样本 ,采用免疫印迹法检测抗ENA抗体 ,经免疫扩散法检测Ig和补体。结果 :抗ENA抗体在自身免疫病中有较高的检出率 ,其中抗平滑肌 (Sm)抗体 ,抗U1 核糖蛋白抗体 (u1RNP)的阳性率在系统性红斑狼疮 (SLE)中分别为 36 .7% ,30 .0 % ;抗SSA和抗SSB抗体的出现在干燥综合征 (SS)患者中分别分4/ 1 2和 2 / 1 2 ;与正常对照和抗体阴性组比较 ,抗ENA抗体阳性血清中IgG、IgM、IgA增高 ,分别为 (2 1 .4±1 4 .8) ,(1 .8± 0 .9) ,(2 .58± 1 .34) g/L ,而C3和C4 水平下降 ,分别为 (1 .1 0± 0 .35) ,(0 .2 3± 0 .1 3) g/L。结论

Objective:To determine the clinical significance of anti-ENA autoantibody profiles and its relations with other immune markers.Methods:Anti-ENA autoantibodies were tested with Western-blot.While immunoglobulins and complements were detected quantitatively by spread.Results:The high postive rate of anti-ENA autoantibody was detected but not specific in several autoimmune diseases.The positive rate of anti-SM and anti-U1RNP autoantibodies were 36.7%, 30.0% in SLE respectively.Anti-SSA and anti-SSB autoantibodies occurred in 33.3% and 16.7% of the patients with SS.The quantity of IgG?IgM and IgA was increased in anti-ENA antibody positive sera wihich were (21.4±14.8),(1.8±0.9),(2.58±1.34)g/L.While the quantity of C3 and C4 was decreased,which were (1.10±0.35),(0.23±0.13)g/L.Conclusion:Humoral immune activation was demonstrated in patients with positive ENA autoantibody.