克拉霉素和乙酰螺旋霉素对铜绿假单胞菌生物被膜的作用

Effects of clarithromycin and acetylspiramycin on Pseudomonas aeruginosa biofilms

目的 :研究克拉霉素 (CAM)、乙酰螺旋霉素 (ASM)对铜绿假单胞菌生物被膜 (BF)合成的影响及其与环丙沙星 (CIP)的相互作用。方法 :色氨酸法测定BF中的多糖蛋白复合物 (GLX) ;高氯酸 蒽酮反应物法定量测定BF的己糖成分 ;噻唑兰法测定活菌数。结果 :1/ 16最低抑菌浓度 (MIC)、1/ 4MIC的CAM可抑制BF中GLX、己糖的合成 ,且可显著增强 1/ 4MIC、1/ 2MIC的CIP对有BF的铜绿假单胞菌的杀菌作用 (P <0 0 5 ) ,而ASM则无以上作用。结论 :CAM可抑制铜绿假单胞菌BF的形成 ,并可增强CIP对有BF的铜绿假单胞菌的杀菌作用 ;

AIM: To study the influence of clarithromycin (CAM) and acetylspiramycin (ASM) on Pseudomonas aeruginosa biofilms (BF) and influence of antibacterial activities between CAM and ciprofloxacin (CIP) on Pseudomonas aeruginosa. METHODS: We measured the glycocalyx (GLX) production by using a L-tryptophan method; quantitatively measured the hexose production of BF by the hyperchloride acid-anthrone reactant method; and determined the viable bacterial counts in BF by the methylthiazaolyldiphenyltetrazolium (MTT) method. RESULTS: CAM had the inhibitory activities on the GLX and hexose production of Pseudomonas aeruginosa at the concentration of 1/16 MIC and 1/4 MIC; after CAM was added, the viable counts in CIP group were decreased significantly (P<0.05). However, acetylspiramycin had no such effect. CONCLUSION: Clarithromycin has the inhibitory activities on BF formation, which enhances the bactericidal action of ciprofloxacin on Pseudomonas aeruginosa within the BF, but acetylspiramycin has no similar effect.