秦巴山区孕妇HCMV感染率高的主要原因初步探析

A preliminary study on the main factor of the high incidence of human cytomegalovirus (HCMV)infection of pregnant women in Qinba mountain area.

目的 :探讨秦巴山区孕妇人巨细胞病毒 (HCMV)感染率高的主要原因 ,初步研究缺锌与HCMV感染的相关性。方法 :ELISA和PCR相结合检测秦巴山区 16 1名孕妇外周血特异性抗CMVIgM抗体 ,CMVDNA ,检测出活动性感染为病例组 ,无活动性感染为对照组。原子吸收光谱法测定其血清锌含量。双抗体夹心ELISA法检测孕妇外周血肿瘤坏死因子α(TNFα)水平。结果 :1.秦巴山区孕妇HCMV活动感染率 19.2 5 % ,明显高于国内外报道。 2 .病例组的血清锌含量明显低于对照组 ,有显著性差异 (P <0 .0 5 )。 3.病例组的TNFα含量与对照组相比有显著差异 (P <0 .0 5 )。 4.对与孕期HCMV活动感染有关的因素 ,进行多元逐步回归分析 ,得出y =0 .375X1(血清锌 ) 0 .34 3X2 (TNFα) - 0 .34 0X3 (胎次 ) +0 .2 0 9X4 (年龄 ) ,血清锌含量与HCMV感染相关性最强 (β=0 .375 ,P <0 .0 1)。 5 .血清锌含量与TNFα含量有显著负相关 (r=- 0 .5 71)。结论 :秦巴山区孕妇HCMV感染率高于国内外报道 ;锌与该地区孕期HCMV活动性感染有明显相关性 。

Objective:To explore the main factor of the high incidence of human cytomegalovirus(HCMV)infection of pregnant women in Qinba mountain area and a preliminary exploration on the relationship between zinc deficiency and cytomegalovirus infection during pregnancy.Methods:CMV-IgM and CMV DAN of 161 pregnant women in Qinba mountain area were detected by ELISA and PCR.30 gravidas were selceted as subject group (SG)who had active infection and 130 gravidas were selected as control group (CG)who had tranquil infection.Serum zinc was determined by atomic absorption spectrophotometry.Serum tumor necrosis factor-α (TNF α)concentration was assessed by ELISA. Results:1.The incidence of HCMV infection of pregnant women in Qinba mountain area was 19.25% which was higher than that in domestic and foreign reports.2.Serum zinc concentration was significantly lower in SG than in CG(P<0.05).3.Serum TNF α concentration was higher in SG than in CG(P<0.05).4.By means of multifactorial regression analysis,the result showed serum zinc had a significantly correlation with HCMV infection.(β=0.375,P<0.01).5.Serum zinc concentration was found to have a negative correlation with TNF α concentration(r=-0.571).Conclusion:The incidence of HCMV infection of pregnant women in QinBa mountain area is significantly higher than that in domestic and foreign reports.The main factor of the high incidence of HCMV infection during pregnancy is zinc deficiency.