石蒜凝集素基因的克隆(英文)

Molecular Cloning of Lectin Gene from Lycoris radiata

用石蒜科植物凝集素基因的保守序列为引物 ,利用RACE PCR技术从石蒜幼嫩叶片中克隆出石蒜凝集素的全长cDNA .通过比较石蒜同其他石蒜科植物凝集素基因序列和推测的氨基酸序列 ,发现石蒜凝集素基因编码一具有信号肽的前体蛋白 .石蒜凝集素同其他石蒜科植物凝集素一样为具有

By the use of RACE PCR technique with RNA extracted from Lycoris radiata young leaves as template and primers specifically designed according to conservative sequences of Amaryllidaceae lectins, the full length cDNA of Lycoris radiata lectin was cloned. Through comparative analysis of Lycoris radiata lectin gene and its deduced amino acid sequence with those of other Amaryllidaceae species,it was found that Lycoris radiata lectin gene encoded a precursor lectin with signal peptide. Lycoris radiata lectin was a mannose binding lectin with three mannose binding boxes like lectins from other Amaryllidaceae species.