谷胱甘肽S-转移酶M1基因型与石棉作业工人血液脂质过氧化水平的关系

The relationship between glutathione S-transferase M1 genotypes and lipid peroxidation in asbestos workers

目的 探讨谷胱甘肽S 转移酶M1(GSTM1)基因型与接触石棉的工人血液发生脂质过氧化的关系。方法 选择 94名石棉作业工人及 5 1名对照工人作为研究对象 ,通过问卷调查收集每个研究对象的一般情况、职业史等。同时测定血浆丙二醛 (MDA)的含量 ,分析淋巴细胞DNA中GSTM1的基因型。结果 石棉作业工人血浆MDA含量为 (0 .2 83± 0 .0 5 4)nmol/L ,明显高于对照组工人 [(0 .16 3± 0 .0 5 3)nmol/L],差异有显著性 (P <0 .0 1) ,但MDA含量与工龄和累积石棉接触剂量之间的相关关系不明显 ;对照组工人携带GSTM1- /-者血浆中的MDA含量 [(0 .190± 0 .0 34 )nmol/L]明显高于携带GSTM1+/+者[(0 .138± 0 .0 5 5 )nmo/L],差异有显著性 (P <0 .0 1) ,而石棉作业工人组虽也有类似趋势 ,但无统计学差异 (P >0 .0 5 ) ;对于石棉作业工人来说 ,在工龄相同或累积石棉接触剂量相同时 ,携带GSTM1- /-者的血浆MDA含量均高于携带GSTM1+/+者 ,但差异均无显著性 (P >0 .0 5 )。结论 接触石棉和GSTM1- /-基因型均与作业工人机体内脂质过氧化有关 ,但石棉的作用可能大于GSTM1基因型的作用。

Objective To study the relationship between glutathione S-transferase M1(GSTM1) genotypes and lipid peroxidation of asbestos workers. Methods 94 asbestos workers and 51 controls were selected as subjects.The general information,occupational history and individual habits were collected by questionnaires in all participants.The venous blood was sampled and the plasma was separated for the detection of malondialdehyde(MDA) level and lymphocytes for DNA isolation and GSTM1 genotyping. Results MDA level was significantly higher in asbestos workers[(0.283±0.054)nmol/L] than that in controls[(0.163±0.053)nmol/L,P<0.01],however,neither duration of exposure nor accumulated asbestos exposure dose was related to MDA levels;MDA levels in control workers with GSTM1-/- genotype[(0.190±0.034)nmol/L] were significantly higher than that in control workers with GSTM1+/+ genotype[(0.138±0.055)nmol/L,P<0.01].Among asbestos workers,the same trend could be found,but the difference was not significant(P>0.05).When the workers were stratified by duration of exposure or accumulated asbestos exposure dose,MDA levels in individuals with GSTM1-/- genotype were also higher than those with GSTM1+/+ genotype,but the differences were also not significant(P>0.05). Conclusion Both exposure to asbestos and deficiency of GSTM1 genotype were related to lipid peroxidation in workers,but the role of the former may be more important than that of the latter.