兔胸部爆炸冲击伤后早期血浆心肌肌钙蛋白Ⅰ含量的变化及意义

Value of changes of plasma cardiac troponin I concentration after early explosive blast injury in rabbits

目的 探讨家兔胸部冲击伤后早期心肌损伤时 ,血浆心肌肌钙蛋白I(cTnI)的变化特点及其诊断意义。方法 采用自动化双位点荧光酶免疫定量检测法 ,观察兔胸部爆炸冲击伤后 5min、30min、3h、6h、12h血浆cTnI水平及心肌组织病理学变化。结果 家兔伤后 30min血浆cTnI水平显著升高 ,与正常对照组相比有统计学意义 (P <0 0 5 ) ,6h达峰值 (P <0 0 1) ,伤后 12h以后又有所下降 ,但仍高于正常对照组 (P <0 0 1)。血浆cTnI水平与心肌损害严重程度密切相关。结论 血浆cTnI水平在胸部爆炸冲击伤后心肌损伤程度的诊断中是一个敏感而特异的指标。

Objective In order to investigate the changes of plasma cardiac troponin I(cTnI)concentration and its diagnostic value for early myocardial injury following thoracic explosive blast injury.Methods Rabbits were subjected to explosive blast injury on their right chest wall.Plasma cTnI concentration was assayed by an immunoassay on the Baxter stratus analyzer in a preliminary research application that uses two cTnI-specific monoclonal antibodies.And histologic changes of myocardium were examined at 1/12,0.5,3,6,12 hours after injury.Results cTnI concentration increased after 30 minutes and reached maximum at 6 hours after injury(P<0 01) cTnI concentration was lower 12 hours after injury but sitll higher than that of normal control(P<0 01) Plasma cTnI concentration was correlated with the severity of myocardial injury.Conclusion Plasma cTnI concentration may be a sensitive and specific marker for the diagnosis of myocardial injury after explosive blast injury.