RP-HPLC法测定穿心莲中穿心莲内酯的含量

Determination of Andrographolide in Herba Andrographis by RP-HPLC

目的 高效液相色谱法测定穿心莲中穿心莲内酯的含量。方法 本品乙醇提取 ,提取物经硅胶柱 ,用氯仿 -甲醇 (9.5∶0 .5 )洗脱 ,洗脱液进行高效液相色谱法分析。色谱柱 μBONDAPAKC18(10 μm ,3.9× 30 0mm) ,流动相 :甲醇 -乙腈 -水 (5 0∶10∶4 0 ) ,流速 :0 .8ml/min ;检测波长 :2 2 5nm。结果 加样平均回收率为 99.2 4 % ,RSD为 0 .2 6 % (n =5 )。结论 实验结果表明 ,该法灵敏度高 ,专属性好 ,操作简便 。

Aim To deuelop an assay method for the determination of Andrographolide in Herba Andrographis by HPLC.Methods The chromatographic conditions were as follows:column μBONDAPAK C 18 (10μm,3.9×300mm);the mobile phase methyl alcohol-acetonitrile-water(50∶10∶40) and detection at 225nm.The flow rate was 0.8ml/min.Results The average recovery was 99.24%(RSD=0.26%,n=5).Conclusion The results showed that the method was simple and accurate in the quality control of Herba Andrographis.