VIDAS CHL检测泌尿生殖道沙眼衣原体感染

Detection of urogenital Chlamydia trachomatis infection by VIDAS CHL assay

目的 探讨VIDASCHL法用于性病患者尿道拭子标本以及男性首段尿 (FCU )标本沙眼衣原体(CT)检测的可行性。方法 使用组织培养法 (TC)、VIDASCHL检测法和聚合酶链反应 (PCR)平行检测男性和女性拭子标本中CT ,以组织培养为金标准 ,对VIDASCHL和PCR进行评价。结果 2 32例男性标本TC阳性49例 ;VIDASCHL和PCR法用于男性尿道拭子标本的敏感性分别为 95 .9%、93.9% ,特异性分别为 95 .6 %、94.5 % ,差异无显著性 ;VIDASCHL法用于男性FCU的敏感性和特异性分别为 85 .7%和 96 .7% ,与拭子标本作自身配对比较 ,检测结果无差异。 15 1例女性拭子标本 ,TC法阳性 2 3例 ,VIDASCHL和PCR法的敏感性分别为 10 0 %和 95 .7% ,特异性分别为 96 .1%、93.8% ,差异无显著性。结论 VIDASCHL法用于性病患者男性和女性拭子标本的CT检测 ,具有很高的敏感性和特异性 ,用于男性患者FCU的CT检测也是可行的 ;对于CT阳性率较高的性病人群 ,VIDASCHL可以不做阻抑证实试验。

Objective To evaluate the VIDAS CHL amydia(CHL) assay for detecting Chlamydia trachomatis with swabs from STD patients and with first catch urine(FCU) specimens of male patients. Methods Tissue culture, VIDAS CHL and PCR were used simultaneously to detect C. trachomatis in the swab specimens of male and female patients. VIDAS CHL and PCR were evaluated with tissue culture as the gold standard. Results Among 232 male specimens 49 were positive by tissue culture. For male swabs, VIDAS CHL and PCR had a sensitivity of 95.9% and 93.9%, and a specificity of 95.6% and 94.5% respectively. For male FCU, VIDAS CHL had a sensitivity of 85.7% and a specificity of 96.7%. Among 151 female specimens 23 were positive by tissue culture; VIDAS CHL and PCR had a sensitivity of 100% and 95.7%, and a specificity of 96.1% and 93.8% respectively. Conclusion VIDAS CHL assay is a very sensitive and specific method for the detection of C. trachomatis with swabs of male and female STD patients and it is also suitable for male FCU specimens. Among population with higher incidence of C. trachomatis infection, CHL Block test may not be necessarily performed.