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大鼠延髓和脊髓背角的PKCγ阳性神经元向丘脑胶状核投射 被引量：2

PKCγ-immunoreactive neurons in medullary and spinal dorsal horns projecting to gelatinosus thalamus nucleus in rats
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摘要 目的 :观察大鼠延髓和脊髓背角内蛋白激酶Cγ亚单位 (PKCγ)阳性神经元向丘脑胶状核的投射。方法 :荧光金 (FG)逆行追踪与PKCγ免疫荧光组织化学染色相结合的双标记技术。结果 :PKCγ阳性神经元主要分布于大鼠延髓和脊髓背角的II层内侧部及II、III层交界处 :将FG注入丘脑胶状核后 ,在延髓背角的I、III层和脊髓背角的I、III、V层及外侧脊核内可见FG标记神经元 ;延髓和脊髓背角I层的部分FG标记神经元呈PKCγ阳性。结论 :大鼠延髓和脊髓背角的PKCγ阳性神经元向丘脑胶状核投射 ,它们可能参与将伤害性刺激信息向丘脑的传递。 Objective:To examine the projection of protein kinase Cγ isoform(PKCγ) immunoreactive neurons in the medullary and spinal dorsal horns to the gelatinosus thalamic nucleus in rats. Methods: Fluoro gold(FG) retrograde tracing method combined with immunofluorescence histochemical staining for PKCγ were used. Results: PKCγ immunoreactive neurons were mainly observed in the inner part of lamina II and the border area between laminae II and III of the medullary and spinal dorsal horns. After injecting FG into the gelatinosus thalamic nucleus, FG retrogradely labelled neurons were mainly found in laminae I, III of the medullary dorsal horn and I, III, V of the spinal dorsal horn and lateral spinal nucleus. Some of these FG labelled neurons in lamina I of the medullary and spinal dorsal horns showed PKC immunoreactivities. Conclusion: PKCγ immunoreactive neurons in the medullary and spinal dorsal horns send projection fibers to the gelatinosus thalamic nucleus in rats. which may be involved in the transmission of nociceptive information from the medullary and spinal dorsal horns to the thalamus.
作者 吴利平 李辉 李云庆 张远强
机构地区 第四军医大学西京医院惠宾病房 第四军医大学解剖学教研室 第四军医大学组织学与胚胎学教研室
出处 《解剖学杂志》 CAS CSCD 北大核心 2002年第2期101-103,共3页 Chinese Journal of Anatomy
基金 国家重点基础研究规划"脑功能和脑重大疾病的基础研究"(G19990 5 4 0 0 0 ) 国家自然科学基金 (39970 2 39 3980 0 0 4 4 3980 0 0 34) 高等学校骨干教师资助计划资助项目
关键词 蛋白激酶Cγ亚单位 延髓背角 脊髓背角 丘脑胶状核 伤害性感受 protein kinase cγ isoform medullary dorsal horn spinal dorsal horn gelatinosus thalamic nucleus nociception rat
分类号 R329 [医药卫生—人体解剖和组织胚胎学]
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参考文献12

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同被引文献19

  • 1倪同上,梅光东,蒋正尧.大鼠坐骨神经切断模型脊髓背角内PKCγ的变化(英文)[J].青岛大学医学院学报,2007,43(1):31-33. 被引量：1
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  • 5MARTIN W J,LIU H,WANG H,et al.Inflammation-induced up-regulation of protein kinase C immunoreactivity in rat spinal cord correlates with enhanced nociceptive processing[J].Neuroscience,1999,88:1267-1269.
  • 6CODERRE T J.Contribution of protein kinase C to central sensitization and persistent pains following tissue injury[J].Neurosci Lett,1992,140:181-183.
  • 7LI Y Q,LI J L,LI H,et al.Protein kinase C gamma-like immunoreactivity of trigeminothalamic neurons in the medullary dorsal horn of the rat[J].Brain Res,2001,913:159-163.
  • 8NI T S,WU S X,LI Y Q.Co-existence of PKCγ and calcium-binding proteins in neurons of the medullary dorsal horn of the rat[J].Neurosignals,2005,11(2):88-91.
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  • 10LI J L,LI Y Q,SAKASHI N,et al.Protein kinase C gamma-like immunoreactivity in the substantia gelatinosa of the medullary dorsal horn of the rat[J].Neurosci Lett,2001,311:159-163.

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解剖学杂志
2002年 第2期

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