ET-1在大鼠纹状体微血管表达的增龄变化

Aging changes of expression of ET-1 on microvessels of rat striatus

目的 :研究内皮素 1 (ET 1 )在纹状体微血管表达的年龄变化 ,为探讨纹状体易卒中机制、揭示脑血管病发病机理提供参考资料。方法 :应用SABC免疫组化染色法及图像分析等方法观测 1～ 2月龄、6～ 7月龄及 >2 4月龄三组共 3 6只Wistar大鼠纹状体微血管ET 1的表达。结果 :随月龄增加 ,ET 1在纹状体、颞叶皮质、海马微血管的表达增加 ,>2 4月龄组大鼠纹状体、海马、颞叶皮质微血管ET 1阳性反应强度与其他两组间存在显著差异 (P <0 .0 5 ) ,同月龄组大鼠纹状体、颞叶皮质、海马微血管的ET 1表达有显著差异 (P <0 .0 5 ) ,其中纹状体阳性反应强度最高。结论 :ET 1的分布及其表达的增龄变化 ,可能与该区易发生脑血管病有关。

Objective:To study the expression of endothelin 1(ET 1) on microvessels of rat striatus so as to provide references for further understanding the etiology and pathogenesis of cerebrovascular disease. Methods: Three different age groups of rats (1 2, 6-7,>24 months old) were studied and determined by streptaridin Biotin peroxidase complex technique and image analysis. Results: The expression of ET 1 on microvessels of striatus, cortex and hippocappus increased with age. Expression of ET 1 in these portions in >24 m rats was significantly higer than that in the other 2 groups(P<0.05). There was a significant difference on the expression of ET 1 in the different portions of the same group(P< 0.05 ). From high to low, they were the striatus, the cortex and the hippocampus. Conclusion: The increase expression of ET 1 on microvessels of caudatoputamen with aging may have important implications for the pathogenesis of cerebrovascular disease in this region.