人骨髓间充质干细胞的培养及多能性研究

Culture and pluripotentiality of human marrow mesenchymal stem cells

目的 培养人骨髓间充质干细胞 (mesenchymalstemcells,MSC)并检测其多能性。方法 抽取人胚胎股骨骨髓 ,Percoll分离液梯度分离后 ,将含MSC的低密度层培养于MSCGM培养基并扩增MSC。将MSC种植于裸鼠皮下 ,4周后观察MSC的分化情况。结果 成功地进行了人MSC的原代和传代培养。植入裸鼠体内后MSC可分化成骨、软骨、脂肪、骨骼肌、肌腱样组织等结构。

Objective To cultivate human mesenchymal stem cells (MSC) derived from fetal bone marrow and examine their pluripotentiality. Methods Bone marrow mononuclear cells from human fetal femur were collected by Percoll gradient centrifugation. The low density cells including MSCs were cultivated and expanded in MSCGM media. The characteristics of the multipotent MSCs were observed by implanting them into nude mice for 4 weeks. Results Human fetal marrow MSCs were successfully cultivated and differentiated in vivo into many kinds of tissues such as bone, cartilage, adipose, skeletal muscle and tendon-like tissue. Conclusion Human fetal marrow MSCs were multipotent stem cells.