赖型钩端螺旋体及澳洲型钩端螺旋体的ompL_1和flaB_2抗原基因序列分析

Sequence analysis of the gene encoding ompL_1 and flaB_2 from Leptospira interrogans serovar lai and Leptospira interrogans serovar australis

目的 构建赖型钩端螺旋体 0 17株及澳洲型钩端螺旋体 6 0 7株外膜蛋白抗原基因om pL1和内鞭毛抗原基因flaB2 的重组质粒 ,并分别对ompL1及flaB2 基因进行序列分析。方法 通过聚合酶链反应扩增ompL1及flaB2 ,并将其分别克隆到pcDNA3.1/Myc His(+)载体T7启动子下游 ,构建抗原基因表达质粒 ,进行序列测定分析。结果 序列分析显示赖型钩体 0 17株与澳洲型钩体 6 0 7株的ompL1相同碱基 94 9个 (98.85 % ) ,碱基变异 11个 (1.15 % ) ;flaB2 的相同碱基 82 3个 (96 .94 % ) ,碱基变异 2 6个(3.0 6 % ) ,呈很高的保守性。结论 赖型钩体 0 17株与澳洲型钩体 6 0 7株的ompL1及flaB2

Objective To construct recombinant plasmids from Leptospira interrogans serovar lai strain 017 and Leptospira interrogans serovar australis strain 607 consisted of an outer membrane protein gene (ompL 1) and a flagellin B gene(flaB 2). The ompL 1 and flaB 2 gene were analyzed by DNA sequencing. Methods The DNA fragments of ompL 1 and flaB 2 were respectively amplified by PCR, then about cloned into the down stream of a T7 promoter of vector pcDNA3.1/Myc His(+), recombinant plasmids of the antigen gene were constructed, and analyzed by DNA sequencing. Results Analysis of the DNA sequence of ompL 1 from L.interrogans lai strain 017 and L.interrogans australis strain 607 showed that the similarity of nucleotide sequence was 98.85%(949/960), variation was 1.15%(11/960); About flaB 2, the similarity was 96.94%(823/849), variation was 3.06%(26/849), the sequences of each were highly conserved. Conclusion Sequences analysis of ompL 1 and flaB 2 from Leptospira interrogans serovar lai strain 017 respectively revealed a high level of identity with that from Leptospira interrogans serovar australis strain 607. [