骨性关节炎关节软骨中Ⅰ型、Ⅱ型、Ⅲ型及Ⅹ型胶原的分布

Distribution of Collagen Types Ⅰ, Ⅱ, Ⅲ and Ⅹ in Osteoarthritic Cartilage

目的 :研究成人骨性关节炎关节软骨中的Ⅰ型、Ⅱ型、Ⅲ型及Ⅹ型胶原的分布。方法 :分别用抗Ⅰ型、Ⅱ型、Ⅲ型及Ⅹ型胶原单克隆抗体对成人骨性关节炎关节软骨切片进行免疫组织化学染色法。结果 :骨性关节炎关节软骨全层无Ⅰ型胶原 ;Ⅹ型胶原分布在深层和钙化层 ;Ⅱ型和Ⅲ型胶原分布相同 ,轻度病变时分布于软骨全层 ,中、重度病变时主要分布于中、深层。结论 :成人骨性关节炎关节软骨中不仅含有Ⅱ型胶原 ,而且含有大量Ⅲ型和Ⅹ型胶原 ,但无Ⅰ型胶原。Ⅹ型胶原和Ⅱ。

Objective To investigate the distribution of collagen types Ⅰ,Ⅱ,Ⅲ and Ⅹ in adult osteoarthritic cartilage. Method Sections of adult osteoarthritic articular cartilage were stained by immunohistochemical method with monoclonal antibodies against collagen types Ⅰ,Ⅱ,Ⅲ and Ⅹ. Results Type I collagen was not detected in osteoarthritic cartilage. Type Ⅹ collagen was detected in deep and calcify layers. Collagen types Ⅱ and Ⅲ were detected through the four layers in mild osteoarthritic cartilage, while in middle and deep layers in modereate to severe osteoarthritic changes. Conclusion There were not only collagen type Ⅱ but also types Ⅲ,Ⅹ in adult osteoarthritic cartilage, otherwise collagen type Ⅰ was not found. Co-distribution of collagen types Ⅱ, Ⅲ and type Ⅹ was detected.