力竭运动对大鼠纹状体、中脑及下丘脑单胺类神经递质含量的影响

Effects of Exhaustion Treadmill Running on Monoamine Neurotransmitters Concentration in Corpus Striatum, Midbrain and Hypothalamus of Rat

为观察力竭性跑台运动对大鼠纹状体、中脑及下丘脑单胺类神经递质含量的影响 ,本研究采用高效液相色谱 -电化学检测法测定了安静时、运动后即刻和运动后 2 4小时大鼠纹状体、中脑及下丘脑多巴胺 (DA)、去甲肾上腺素 (NE)和 5 -羟色胺 (5 -HT)水平。结果显示 ,力竭运动后即刻下丘脑DA水平显著降低 ,运动后中脑NE水平和运动后 2 4小时纹状体的NE水平升高 ;运动后纹状体和下丘脑内 5 -HT有升高的趋势 ;中脑的DA/ 5 -HT比值在运动后显著性升高 ,而在下丘脑中则显著性下降。提示 ,大鼠运动至力竭时 ,中枢神经系统的抑制是多种递质共同作用的结果 ;运动导致的中枢神经系统的抑制过程并不是在全脑均衡发生的 ,中脑表现一定的兴奋性作用 ,而在下丘脑则表现为抑制 ;DA/ 5 -HT的比值是评定中枢神经系统疲劳的敏感性指标 。

This study focuses on the changes in dopamine (DA), norepinephrine (NE) and 5-hydroxytryptamine (5-HT)concentration in corpus striatum of rat, midbrain and hypothalamus after exhaustion treadmill running, to provide facts and recommendations for exercise-induced central fatigue studies. High-performance liquid chromatography with electrochemical detection was used for evaluating the amounts of DA, NE and 5-HT. DA in the hypothalamus was decreased significantly than that in the sedentary group after exhaustion treadmill running. NE levels in the midbrain were significantly higher than that in the sedentary group after exhaustion treadmill running and returned to sedentary levels 24h later. 5-HT levels in the corpus striatum and hypothalamus showed an increasing trend. The ratio of DA/5-HT decreased significantly right after running in midbrain, while increased significantly in hypothalamus. The results show that brain inhibition occurs due to the change in neurotransmitters after exhaustion treadmill running. Brain inhibition is not symmetrical in all the area of the brain. The midbrain shows some degrees of excitation, the hypothalamus shows inhibition. The ratio of DA/5-HT is a sensitive index in evaluating exercise-induced central fatigue.