子宫内膜癌中p16基因缺失及CpG岛甲基化的研究

p16 gene deleton and 5’CpG island methylation in endometrial carcinoma

目的 研究子宫内膜癌中 p16基因缺失及CpG岛甲基化情况。 方法 用PCR技术检测 p16基因在子宫内膜癌中的纯合性缺失及第一外显子异常甲基化。结果 36例癌组织中 9例发生基因缺失 ,12例发生甲基化 ,未发现基因缺失与甲基化同时存在的病例。结论 1.p16基因缺失与子宫内膜癌组织学分级严重程度和临床分期呈正相关 ;2 .5’CpG甲基化可能是 p16基因失活的重要机理 ,参与子宫内膜癌发生、发展。

Objective To study p16 gene deletion and 5 CpG island methylation in endometrial carcinoma. Methods The p16 gene deletion and 5 CpG island methylation were determined in endometrial carcinoma and benign endometrium by PCR technique. Results The p16 gene deletion and 5 CpG island methylation rates in endometrial carcinoma were 25.00%(9/36)and 33.33% (12/36) respectively. Conclusion 1. p16 gene deletion was positively correlated with pathological grades and clinical stages in endometrial carcinoma. 2. p16 gene methylation was an important event in the development of endometrial carcinoma.