摘要
本文报道用薄层扫描法测定血浆中硫酸奎丁尼浓度,方法简便灵敏。血浆样品用氯仿提取后,直接点于硅胶GF_(254)板上,以环己烷:氯仿:二乙胺(5∶4∶2)为展开剂,斑点于岛津CS-930扫描仪在λs=237nm,λ_R=257nm处进行扫描。在0.4μg～8.0μg之间,线性关系良好,平均回收率为98.78%。为临床监测硫酸奎尼丁的血药浓度及动力学研究提供了可行方法。
A simple and sensitive method for determinatingthe concentration of quinidine sulfate in Plasma byTLC is presented Plasma samples were extrced withCHCI_3, spotted directly on the silica GF_(254). Plate and de-veloped with cyelohexane--chloroform--diethylamine(5: 4: 2). The spots were scaned on a shimadzaseanner CS--930 atλ_S=237 nm λ_R=257 nm A goodlincar correlation was obtained in the range of 0.4～0. 8 μg The average recovery was 98. 78%. It providesa reliable method for clinical determination of quinidinesulfate concentration in plasma and kinetics study.
出处
《西安医科大学学报》
CSCD
1991年第1期84-86,共3页
Journal of Xi'an Medical University(Chinese)
关键词
薄层扫描
硫酸奎尼丁
血浆浓度
thin layer chromatographic densitometry
quinidine sulfate
plasma concentration
