胎儿食管粘膜上皮细胞DNA含量的细胞光度术及细胞增殖分析

CYTOPHOTOMETRIC DETERMINATION OF DNA CONTENT OF ESOPHAGEAL MUCOSA CELLS OF HUMAN FETUS WITH DIFFERENT MONTHLY AGE AND ANALYSIS OF PROLIFERATION OF CELLS IN TISSUES

本文采用Feulgen-DNA含量细胞分光光度术动态地测量了林县不同胎龄人的食管上皮细胞DNA含量及其染色体倍性的精确变化,DNA含量及相关细胞频数关系用直方图显示吸收光度量。结论是:胎龄愈高,DNA含量愈集中分布,最大峰值的G_1的造型愈高,DNA值的标准差(s)和标准误(s_?)愈小。DNA含量在2～4C范围,符合一个增殖细胞群体的特征。食管上皮的增殖和分化情况由细胞分光光度计所测量的DNA含量的分级依据和细胞形态学特征所反映,同时探讨了在增殖与分化“失常”的微细形态学改变之前可能显示出精微的DNA含量的改变。

By Fculgen--DNA cytophotometry itself weredynamically determined the DNA content of esophagealmucous mermbrane epithclial cells from human em-bryocs with different ages in Lin County (the district ofthe precailing esophagcal cancer) and correspondingchanges with multiples of the chromosome value ASwas shown by the histogram bearing on the DNA con-tent and on the relative cell frequencies we have cometo conclusion that the more the age of embryo in-creases, the more concentrates distribution of DNAcontent, the higher is the shapes of summit peak G_1,the smaller are the standard deviation (S) and standarderror(S_x)of DNA value The DNA content of the mu-cous membrane cells distributes in amount 2C～4C,forming a prolifcous cycle of a growing cell populationThe status of the proliferation and differetiation ofesophageal epithalium is indicated by thecytophotometrical evidence of the classes of DNA con-tent as well as the cytomorphological features. The pos-sible detective minute changes of DNA content beforethe minute mophological changes abnormalproliferation and differentiation were surveyed in this pa-per